The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

Nucleotide

• Quickly search for highly similar sequences (megablast)
• Quickly search for divergent sequences (discontiguous megablast)
• Nucleotide-nucleotide BLAST (blastn)
• Search for short, nearly exact matches
• Search trace archives with megablast or discontiguous megablast

Protein

• Protein-protein BLAST (blastp)
• Position-specific iterated and pattern-hit initiated BLAST (PSI- and PHI-BLAST)
• Search for short, nearly exact matches
• Search the conserved domain database (rpsblast)
• Protein homology by domain architecture (cdart)

Translated

• Translated query vs. protein database (blastx)
• Protein query vs. translated database (tblastn)
• Translated query vs. translated database (tblastx)

Genomes

• Human, mouse, rat, chimp, cow, pig, dog, sheep, cat
• Chicken, puffer fish, zebrafish
• Fly, honey bee, other insects
• Microbes, environmental samples
• Plants, nematodes
• Fungi, protozoa, other eukaryotes

Special

• Search for gene expression data (GEO BLAST)
• Align two sequences (bl2seq)
• Screen for vector contamination (VecScreen)
• Immunoglobin BLAST (IgBlast)
• SNP BLAST

Meta

• Retrieve results