Mon Oct 26 13:49:22 EST 1998 iteralign.c - Program for the alignment of multiple protein sequences. iteralign -s kin10.fasta Sequences from: kin10.fasta Scoring matrix: BLOSUM62 High threshold probability: 0.010 High threshold dropoff: 0.500 Low threshold probability: 0.500 Low threshold dropoff: 0.500 Low threshold weight in consensus: 0.500 Sequence weights based on SSPA scores between sequences normalized by minimum self-score Sequences clustered by similarity (single linkage clustering) Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 13 12 13 6 5 7 10 8 12 2 SKH SKH H - 27 7 10 7 10 8 13 11 3 APK CAPK .... - 14 15 13 10 12 12 12 4 GFR EGFR - 28 24 19 16 13 9 5 FES THIS IS .......... - 27 17 14 13 6 6 DGM PDGF RE - 18 14 10 7 7 AF1 RAF1 ................ - 15 8 12 8 MOS CMOS - 11 10 9 EE1 WEE1 ...................... - 10 10 SVK HSVK - Average similarity: 12.6 Sequence Length Weight 1 D28 CD28 293 1.037 2 SKH SKH H 263 1.009 3 APK CAPK 255 0.983 4 GFR EGFR 264 0.961 5 FES THIS IS 271 0.974 6 DGM PDGF RE 340 0.984 7 AF1 RAF1 261 0.998 8 MOS CMOS 286 1.005 9 EE1 WEE1 280 1.020 10 SVK HSVK 263 1.030 Average length: 277.6 Sequence 1 2 3 4 5 6 7 8 9 10 eps time Cons.diff. Aligned pos. Iteration 1 5 7 6 6 4 4 5 6 5 5 43" 101.1 150 Iteration 2 6 3 3 6 4 4 4 4 20 3 55" 32.9 172 Iteration 3 6 4 2 3 3 6 2 3 2 4 25" 14.3 184 Iteration 4 3 2 2 2 2 2 3 1 2 2 15" 2.0 188 Iteration 5 1 1 1 2 1 1 1 1 1 1 8" 0.2 188 Iteration 6 2 1 1 1 1 1 1 1 1 1 7" 0.1 188 Iteration 7 1 1 1 1 1 1 1 1 2 1 10" 0.1 188 Iteration 8 1 1 1 1 1 1 1 2 1 1 9" 0.1 188 Iteration 9 1 1 1 1 2 1 1 1 1 1 7" 0.1 188 Iteration 10 1 1 1 1 1 1 1 1 1 1 7" 0.0 188 Total time after iterations = 3' 8" ********************************************************* BLOCKS OF SIMILARITY BETWEEN SEQUENCES Blocks are defined as at least 3 consecutive positions of at least 6 aligned sequences *************************************************** CONSENSUS BLOCKS Lower case indicates positions of CI < 0.0 Numbers in parentheses indicate the range of insertion lengths between successive blocks. block # of seqs. number in block (8-23) 1. 9 LGSGSFGeVYKA (11-20) 2. 9 EtTkPKekqRLLNE (0-11) 3. 9 VRILRRL (0-1) 4. 9 RHPNIVRLM (7-25) 5. 8 LYMVMELCeG (0-3) 6. 10 GDLfDYLR (15-114) 7. 10 QVAnGMdYLHSKnIIHRDLKPENILInEPG (1-4) 8. 10 IKIGDFGLAR (13-19) 9. 10 EWmAPE (6-9) 10. 10 YTtqADVWSFGIVIWEM (0-8) 11. 10 SAPY (3-18) 12. 10 QIY (7-15) 13. 8 PeL (23-36) 14. 9 ERL (0-27) *************************************************** PATTERNS x indicates positions of CI < 0.0 Lower case indicates residues less frequent than 20% in the aligned column. Only residues appearing in an alignment column with frequency greater than 5% are indicated. Numbers in parentheses indicate the range of insertion lengths between successive blocks. block # of seqs. number in block (8-23) 1. 9 [LIv]G[SReat]G[SAtegn][Fy][GS]xV[YFVm][Kqrels][AVG] (11-20) 2. 9 [EDKs]x[TSAvl]x[Pysl][KDres]xxx[RAEhk][LVFti][Leicrm][SQNvd] [Ek] (0-11) 3. 9 [AVLik][RSnaky][VIL][Lqam][RKqa][RAekqs][LVty] (0-1) 4. 9 [RDkgns][Hdfn][PDav][NHaf][Ilv][VLic][Rpeqlk][Lvf] [MVLyei] (7-25) 5. 8 [LVi][Yhaq][MLIq][Vqi][MTfvi][EQy][Lfgwy][CAVlm]x[Gnf] (0-3) 6. 10 [Gls][DSec][Lamf]x[DRTeks][YHfr][Lmicv][REhi] (15-114) 7. 10 [Qdem][VLita][ALvc]x[Gat][MVLif]x[YF][LIc][Hea][SArhd] [KQlhr]x[Icly][IVlt][Hy][RL]D[Lim][KA][PAts][ERAqn]N[ILVc] [LFm][Ivyl]x[Etkfhq][PQdek][Gednq] (1-4) 8. 10 [LIVc][Kciq][ILv][GTsc]DFG[Lmacf][As][RKcse] (13-19) 9. 10 [EKdtl][WYth]xA[Pl]E (6-9) 10. 10 [Yvf][TSdn]xx[VSA]D[VItmw][WYf][Sa][LFyia][Ga][IVcl][TLIV] [LIvfa][WYFa][Eiq][MLTai] (0-8) 11. 10 [Asrtqeg][Akmlts][Png][YIrf] (3-18) 12. 10 [Qrie][ILvsft][Yfhwirs] (7-15) 13. 8 Px[Lsvhi] (23-36) 14. 9 [EDQta][RLkae][Lyvi] (0-27) ********************************************************* MULTIPLE ALIGNMENT OF SEQUENCES NOT aligned positions are in lower case and are flushed to the left as much as possible, to keep the output as compact as possible without overlapping aligned positions. Aligned positions are in upper case. Conservation indicates the range of CI values for block columns: 1 : perfect conservation, CI=1.0; * : CI >= 0.40; - : CI < 0.0; 1 11 21 31 41 CONSENSUS LGSGSFGEVYKA D28 CD28 1 anykrlek VGEGTYGVVYKA ldlrpgqgqrv 31 SKH SKH H 1 akydikal IGRGSFSRVVRV ehratrqpyaikm 33 APK CAPK 1 dqferikt LGTGSFGRVMLV khmetgnhyamki 33 GFR EGFR 1 tefkkikv LGSGAFGTVYKG lwipegekvkipv 33 FES THIS IS 1 vlnravpkdkwvlnhedlvlgeq IGRGNFGEVFSG rlradntlvavks 48 DGM PDGF RE 1 dqlvlgrt LGSGAFGQVVEA tahglshsqatmk 33 AF1 RAF1 1 sevmlstr IGSGSFGTVYKG kwhgdvavkilkv 33 MOS CMOS 1 eqvcllqr LGAGGFGSVYKA tyrgvpvaikqvn 33 EE1 WEE1 1 trfrnvtl LGSGEFSEVFQV edpvektlkyavk 33 SVK HSVK 1 mgftihgaltpgsegcvf 18 *1 1 **-1 51 61 71 81 91 CONSENSUS ETTKPKEKQRLL NE VRILRRL RHPN D28 CD28 32 VAL K KIRLE SE degvpstaire ISLLKEL KDDN 64 SKH SKH H 34 i E TKYREGREVCeSE LRVLRRV RHAN 59 APK CAPK 34 ldkq KVVKLKQIEHTL NE KRILQAV NFPF 62 GFR EGFR 34 aikelr EATSPKANKEIL DE AYVMASV DNPH 64 FES THIS IS 49 cr ETLPPDIKAKFL QE AKILKQY SHPN 75 DGM PDGF RE 34 vavkmlk STARSSEKQALM SE ly 56 AF1 RAF1 34 v DPT PEQFQAFR NE VAVLRKT RHVN 58 MOS CMOS 34 kctknrl asrrsfwae LNVA RL RHDN 59 EE1 WEE1 34 klkv KFSGPKERNRLL QE VSIQRAL k GHDH 63 SVK HSVK 19 DSSHPDYPQRVI VK agwytstshe ARLLRRL DHPA 53 - - --- * * 101 111 121 131 141 CONSENSUS IVRLM LYMVMELCEG GDL D28 CD28 65 IVRLY divhsdahk LYLVFEFLD LDL 90 SKH SKH H 60 IIQLV evfetqer VYMVMELATG GEL 85 APK CAPK 63 LVKLE fsfkdnsn LYMVMEYVPG GEM 88 GFR EGFR 65 VCRLL gicltst VQLITQLMPF GCL 89 FES THIS IS 76 IVRLI gvctqkqp IYIVMELVQG GDF 101 DGM PDGF RE 57 GDL 59 AF1 RAF1 59 ILLFM gymtkdn LAIVTQWCEG SSL 83 MOS CMOS 60 IVRVV aastrtpagsnslgtiimefggnvt LHQVIYGAAG hpe GDA 105 EE1 WEE1 64 IVELM dswehggf LYMQVELCEN GSL 89 SVK HSVK 54 ILPLL dlhvvsgvtclvlpkyqa DL 78 ** * * * * - * * 151 161 171 181 191 CONSENSUS FD YL R D28 CD28 91 KR YM E gipkdqplgadivkkfmm 113 SKH SKH H 86 FD RI I akgsfterdatrvlq 105 APK CAPK 89 FS HL R rigrfsepharfyaa 108 GFR EGFR 90 LD YV R ehkdnigsqyllnwcv 110 FES THIS IS 102 LT FL R tegarlrmktllqmvg 122 DGM PDGF RE 60 VD YL H rnkhtflqrhsnkhcppsaelysnalpvgfslpshlnltges 106 AF1 RAF1 84 YK HL H vqetkfqmfqlidiar 104 MOS CMOS 106 GEpHC R tggqlslgkclkysl 126 EE1 WEE1 90 DR FL E eqgqlsrldefrvwkilv 112 SVK HSVK 79 YT YLsR rlnplgrpqiaavsr 99 - * 201 211 221 231 241 CONSENSUS D28 CD28 114 SKH SKH H 106 APK CAPK 109 GFR EGFR 111 FES THIS IS 123 DGM PDGF RE 107 dggymdmskdesidyvpmldmkgdikyadiespsymapydnyvpsapert 156 AF1 RAF1 105 MOS CMOS 127 EE1 WEE1 113 SVK HSVK 100 251 261 271 281 291 CONSENSUS QVANGMDYLHSKNIIHRDLKPENILIN D28 CD28 114 QLCKGIAYCHSHRILHRDLKPQNLLIN 140 SKH SKH H 106 MVLDGVRYLHALGITHRDLKPENLLYY 132 APK CAPK 109 QIVLTFEYLHSLDLIYRDLKPENLLID 135 GFR EGFR 111 QIAKGMNYLEDRRLVHRDLAARNVLVK 137 FES THIS IS 123 DAAAGMEYLESKCCIHRDLAARNCLVT 149 DGM PDGF RE 157 yratlindspvlsytdlvgfsy QVANGMDFLASKNCVHRDLAARNVLIC 205 AF1 RAF1 105 QTAQGMDYLHAKNIIHRDMKSNNIFLH 131 MOS CMOS 127 DVVNGLLFLHSQSIVHLDLKPANILIS 153 EE1 WEE1 113 EVALGLQFIHHKNYVHLDLKPANVMIT 139 SVK HSVK 100 QLLSAVDYIHRQGIIHRDIKTENIFIN 126 -* -*** - ***1** 1***- 301 311 321 331 341 CONSENSUS EPG IKIGDFGLAR EW MAPE D28 CD28 141 KDG n LKLGDFGLAR afgvplraytheivtl WyRAPE vl 178 SKH SKH H 133 HPG tdsk IIITDFGLAS arkkgddclmkttcgtp EY IAPE vl 174 APK CAPK 136 QQG y IQVTDFGFAK rvkgrtwtlcgtp EY LAPE ii 170 GFR EGFR 138 TPQ h VKITDFGLAK llgaeekeyhaeggkvpi KW MALE si 177 FES THIS IS 150 EKN v LKISDFGMSR eaadgiyaasgglrqvpv KW TAPE al 189 DGM PDGF RE 206 E G kl VKICDFGLAR dimrdsnyiskgstylpl KW MAPE si 245 AF1 RAF1 132 E G lt VKIGDFGLA tvksrwsgsqqveqptgsv LW MAPE vi 171 MOS CMOS 154 EQD v CKISDFGCSE kledllcfqtpsyplggty TH RAPE ll 194 EE1 WEE1 140 FEG t LKIGDFGMA svwpvprgmeregdc EY IAPE vl 175 SVK HSVK 127 TPE d ICLGDFGAAC fvqgsrsspfpygiagti DT NAPE vl 166 * * 111 * -1*1 351 361 371 381 391 CONSENSUS YTTQADVWSFGIVIWEM SAPY D28 CD28 179 lggkq YSTGVDTWSIGCIFAEM cn RKPI fsgdseid 214 SKH SKH H 175 vrkp YTNSVDMWALGVIAYIL lsg TMPF eddnrt 208 APK CAPK 171 lskg YNKAVDWWALGVLIYEM AAGY ppffadqpi 204 GFR EGFR 178 lhri YTHQSDVWSYGVTVWEL mtfg SKPY dgipas 212 FES THIS IS 190 nygr YSSESDVWSFGILLWET fslg ASPY pnlsnq 224 DGM PDGF RE 246 fnsl YTTLSDVWSFGILLWEI ftlg GTPY pelpmnd 281 AF1 RAF1 172 rmqdnnp FSFQSDVYSYGIVLYEL mtg ELPY srd 205 MOS CMOS 195 kgeg VTPKADIYSFAITLWQM ttk QAPY sgerqh 228 EE1 WEE1 176 anhl YDKPADIFSLGITVFEA AANI vlpdng 206 SVK HSVK 167 agdp YTTTVDIWSAGLVIFET avhnaslf SAPR gpkrgpcdsq 209 * -- 1 ** ** * * 401 411 421 431 441 CONSENSUS QIY PEL D28 CD28 215 QIF kifrvlgtpneaiwp divylpdfkpsfpqwrr 249 SKH SKH H 209 RLY rqilrgkysysgepw PSV snlakdfidrlltvdpg 246 APK CAPK 205 QIY ekivsgkvrfpshfs sdlkdllrnllqvdltk 239 GFR EGFR 213 EIS silekgerlpq PPI ctidvymimvkcwmida 246 FES THIS IS 225 QTR efvekggrlpc PEL cpdavfrlmeqcwayep 258 DGM PDGF RE 282 QFY naikrgyrmaq PAH asdeiyeimqkcweekf 315 AF1 RAF1 206 QII fmvgrgyas PDL sklykncpkamkrlvad 237 MOS CMOS 229 ILY avvaydlr PSL saavfedslpgqrlgdv 259 EE1 WEE1 207 QSW qklrsgdlsda PRL sstdngssltsssretp 240 SVK HSVK 210 itriirqa QVH vdefsph PES rltsryrsraagnnrpp 247 * 1- 451 461 471 481 491 CONSENSUS ERL D28 CD28 250 kdlsqvvpsldprgidll DKL laydpinrisarraaihpyfqes 293 SKH SKH H 247 armtal QAL rhpwvvsm 263 APK CAPK 240 rfgnlkdgvndiknhk 255 GFR EGFR 247 dsrpkfr ELI iefskmar 264 FES THIS IS 259 gqrpsfsaiy QEL 271 DGM PDGF RE 316 etrppfsqlvlll ERL lgegykkky 340 AF1 RAF1 238 cvkkvkeerplfpqilssi ELL qh 261 MOS CMOS 260 iqrcwrpsaaqrps ARL llvdltslka 286 EE1 WEE1 241 ansiigqggl DRV vewmlspeprnrptidqilatdevcw 279 SVK HSVK 248 ytrpaw TRY ykmdidv 263 * 501 CONSENSUS D28 CD28 SKH SKH H APK CAPK GFR EGFR FES THIS IS DGM PDGF RE AF1 RAF1 MOS CMOS EE1 WEE1 280 v 280 SVK HSVK ALIGNED SEQUENCES: >D28 CD28 anykrlek----------------VGEGTYGVVYKA-ldlrpgqgqrv-----------V AL-K-KIRLE-SE-degvpstaire-ISLLKEL---KDDNIVRLY-divhsdahk----- ------------LYLVFEFLD------LDLKR-YM-E-gipkdqplgadivkkfmm---- ------------------------------------------------------------ ---------------------------------QLCKGIAYCHSHRILHRDLKPQNLLIN KDG-n----LKLGDFGLAR-afgvplraytheivtl-----WyRAPE-vllggkq---YS TGVDTWSIGCIFAEM-cn-------RKPI-fsgdseid-----------QIF-kifrvlg tpneaiwp-----divylpdfkpsfpqwrrkdlsqvvpsldprgidll--DKL-laydpi nrisarraaihpyfqes---- >SKH SKH H akydikal----------------IGRGSFSRVVRV-ehratrqpyaikmi-------E- TKYREGREVCeSE-------------LRVLRRV---RHANIIQLV-evfetqer------ ------------VYMVMELATG-----GELFD-RI-I-akgsfterdatrvlq------- ------------------------------------------------------------ ---------------------------------MVLDGVRYLHALGITHRDLKPENLLYY HPG-tdsk-IIITDFGLAS-arkkgddclmkttcgtp---EY-IAPE-vlvrkp----YT NSVDMWALGVIAYIL-lsg------TMPF-eddnrt-------------RLY-rqilrgk ysysgepw-PSV-snlakdfidrlltvdpgarmtal--------------QAL-rhpwvv sm------------------- >APK CAPK dqferikt----------------LGTGSFGRVMLV-khmetgnhyamkildkq----KV VKLKQIEHTL-NE-------------KRILQAV---NFPFLVKLE-fsfkdnsn------ ------------LYMVMEYVPG-----GEMFS-HL-R-rigrfsepharfyaa------- ------------------------------------------------------------ ---------------------------------QIVLTFEYLHSLDLIYRDLKPENLLID QQG-y----IQVTDFGFAK-rvkgrtwtlcgtp-------EY-LAPE-iilskg----YN KAVDWWALGVLIYEM----------AAGY-ppffadqpi----------QIY-ekivsgk vrfpshfs-----sdlkdllrnllqvdltkrfgnlkdgvndiknhk-------------- --------------------- >GFR EGFR tefkkikv----------------LGSGAFGTVYKG-lwipegekvkipvaikelr--EA TSPKANKEIL-DE-------------AYVMASV---DNPHVCRLL-gicltst------- ------------VQLITQLMPF-----GCLLD-YV-R-ehkdnigsqyllnwcv------ ------------------------------------------------------------ ---------------------------------QIAKGMNYLEDRRLVHRDLAARNVLVK TPQ-h----VKITDFGLAK-llgaeekeyhaeggkvpi--KW-MALE-silhri----YT HQSDVWSYGVTVWEL-mtfg-----SKPY-dgipas-------------EIS-silekge rlpq-----PPI-ctidvymimvkcwmidadsrpkfr-------------ELI-iefskm ar------------------- >FES THIS IS vlnravpkdkwvlnhedlvlgeq-IGRGNFGEVFSG-rlradntlvavkscr------ET LPPDIKAKFL-QE-------------AKILKQY---SHPNIVRLI-gvctqkqp------ ------------IYIVMELVQG-----GDFLT-FL-R-tegarlrmktllqmvg------ ------------------------------------------------------------ ---------------------------------DAAAGMEYLESKCCIHRDLAARNCLVT EKN-v----LKISDFGMSR-eaadgiyaasgglrqvpv--KW-TAPE-alnygr----YS SESDVWSFGILLWET-fslg-----ASPY-pnlsnq-------------QTR-efvekgg rlpc-----PEL-cpdavfrlmeqcwayepgqrpsfsaiy----------QEL------- --------------------- >DGM PDGF RE dqlvlgrt----------------LGSGAFGQVVEA-tahglshsqatmkvavkmlk-ST ARSSEKQALM-SE-ly-------------------------------------------- ---------------------------GDLVD-YL-H-rnkhtflqrhsnkhcppsaely snalpvgfslpshlnltgesdggymdmskdesidyvpmldmkgdikyadiespsymapyd nyvpsapertyratlindspvlsytdlvgfsy-QVANGMDFLASKNCVHRDLAARNVLIC E-G-kl---VKICDFGLAR-dimrdsnyiskgstylpl--KW-MAPE-sifnsl----YT TLSDVWSFGILLWEI-ftlg-----GTPY-pelpmnd------------QFY-naikrgy rmaq-----PAH-asdeiyeimqkcweekfetrppfsqlvlll-------ERL-lgegyk kky------------------ >AF1 RAF1 sevmlstr----------------IGSGSFGTVYKG-kwhgdvavkilkvv-------DP T-PEQFQAFR-NE-------------VAVLRKT---RHVNILLFM-gymtkdn------- ------------LAIVTQWCEG-----SSLYK-HL-H-vqetkfqmfqlidiar------ ------------------------------------------------------------ ---------------------------------QTAQGMDYLHAKNIIHRDMKSNNIFLH E-G-lt---VKIGDFGLA--tvksrwsgsqqveqptgsv-LW-MAPE-virmqdnnp-FS FQSDVYSYGIVLYEL-mtg------ELPY-srd----------------QII-fmvgrgy as-------PDL-sklykncpkamkrlvadcvkkvkeerplfpqilssi-ELL-qh---- --------------------- >MOS CMOS eqvcllqr----------------LGAGGFGSVYKA-tyrgvpvaikqvnkctknrl--- --------------asrrsfwae---LNVA-RL---RHDNIVRVV-aastrtpagsnslg tiimefggnvt-LHQVIYGAAG-hpe-GDAGEpHC-R-tggqlslgkclkysl------- ------------------------------------------------------------ ---------------------------------DVVNGLLFLHSQSIVHLDLKPANILIS EQD-v----CKISDFGCSE-kledllcfqtpsyplggty-TH-RAPE-llkgeg----VT PKADIYSFAITLWQM-ttk------QAPY-sgerqh-------------ILY-avvaydl r--------PSL-saavfedslpgqrlgdviqrcwrpsaaqrps------ARL-llvdlt slka----------------- >EE1 WEE1 trfrnvtl----------------LGSGEFSEVFQV-edpvektlkyavkklkv----KF SGPKERNRLL-QE-------------VSIQRAL-k-GHDHIVELM-dswehggf------ ------------LYMQVELCEN-----GSLDR-FL-E-eqgqlsrldefrvwkilv---- ------------------------------------------------------------ ---------------------------------EVALGLQFIHHKNYVHLDLKPANVMIT FEG-t----LKIGDFGMA--svwpvprgmeregdc-----EY-IAPE-vlanhl----YD KPADIFSLGITVFEA----------AANI-vlpdng-------------QSW-qklrsgd lsda-----PRL-sstdngssltsssretpansiigqggl----------DRV-vewmls peprnrptidqilatdevcwv >SVK HSVK mgftihgaltpgsegcvf----------------------------------------DS SHPDYPQRVI-VK-agwytstshe--ARLLRRL---DHPAILPLL-dlhvvsgvtclvlp kyqa------------------------DLYT-YLsR-rlnplgrpqiaavsr------- ------------------------------------------------------------ ---------------------------------QLLSAVDYIHRQGIIHRDIKTENIFIN TPE-d----ICLGDFGAAC-fvqgsrsspfpygiagti--DT-NAPE-vlagdp----YT TTVDIWSAGLVIFET-avhnaslf-SAPR-gpkrgpcdsqitriirqa-QVH-vdefsph ---------PES-rltsryrsraagnnrppytrpaw--------------TRY-ykmdid v-------------------- *********************************************** BLOCKS OF ALIGNED SEQUENCES: Consensus and Sequence Positions. A subdomain is defined as consecutive blocks including the same set of sequences where the separations between blocks in at least 90% of the included sequences is no more than 2 residues Summary statistics. Block: 1. Size: 9; len: 12; Ave CI: 0.50; Ave Seq Sco: 3.42 Block: 2. Size: 9; len: 14; Ave CI: 0.10; Ave Seq Sco: 1.64 Block: 3. Size: 9; len: 7; Ave CI: 0.26; Ave Seq Sco: 2.00 Block: 4. Size: 9; len: 9; Ave CI: 0.30; Ave Seq Sco: 2.67 Block: 5. Size: 8; len: 10; Ave CI: 0.28; Ave Seq Sco: 2.40 Block: 6. Size: 10; len: 8; Ave CI: 0.24; Ave Seq Sco: 2.38 Block: 7. Size: 10; len: 30; Ave CI: 0.38; Ave Seq Sco: 2.93 Block: 8. Size: 10; len: 10; Ave CI: 0.57; Ave Seq Sco: 3.50 Block: 9. Size: 10; len: 6; Ave CI: 0.51; Ave Seq Sco: 3.50 Block: 10. Size: 10; len: 17; Ave CI: 0.40; Ave Seq Sco: 3.18 Block: 11. Size: 10; len: 4; Ave CI: 0.22; Ave Seq Sco: 2.50 Block: 12. Size: 10; len: 3; Ave CI: 0.22; Ave Seq Sco: 2.67 Block: 13. Size: 8; len: 3; Ave CI: 0.37; Ave Seq Sco: 3.00 Block: 14. Size: 9; len: 3; Ave CI: 0.24; Ave Seq Sco: 2.33 Block 1. Size: 9; Length: 12; Average CI: 0.50; 1 11 CONSENSUS LGSGSFGEVY KA Score/pos. Ins. 1 D28 CD28 9 VGEGTYGVVY KA 20 3.33 11 2 SKH SKH H 9 IGRGSFSRVV RV 20 2.33 14 3 APK CAPK 9 LGTGSFGRVM LV 20 2.83 17 4 GFR EGFR 9 LGSGAFGTVY KG 20 3.92 19 5 FES THIS IS 24 IGRGNFGEVF SG 35 3.17 15 6 DGM PDGF RE 9 LGSGAFGQVV EA 20 3.58 20 7 AF1 RAF1 9 IGSGSFGTVY KG 20 4.00 14 8 MOS CMOS 9 LGAGGFGSVY KA 20 4.00 29 9 EE1 WEE1 9 LGSGEFSEVF QV 20 3.25 17 10 SVK HSVK Block 2. Size: 9; Length: 14; Average CI: 0.10; 1 11 CONSENSUS ETTKPKEKQR LL-NE Score/pos. Ins. 1 D28 CD28 32 VAL K KIR LE SE 42 0.36 11 2 SKH SKH H 35 E TKYREGRE VCeSE 48 1.14 0 3 APK CAPK 38 KVVKLKQIEH TL NE 51 1.64 0 4 GFR EGFR 40 EATSPKANKE IL DE 53 2.36 0 5 FES THIS IS 51 ETLPPDIKAK FL QE 64 1.79 0 6 DGM PDGF RE 41 STARSSEKQA LM SE 54 2.29 ly 7 AF1 RAF1 35 DPT PEQFQA FR NE 47 1.36 0 8 MOS CMOS 9 EE1 WEE1 38 KFSGPKERNR LL QE 51 2.43 0 10 SVK HSVK 19 DSSHPDYPQR VI VK 32 1.14 10 ****************************************************** Blocks 3-4 are part of a Subdomain. Block 3. Length: 7; Average CI: 0.26; Block 4. Length: 9; Average CI: 0.30; Total: Size: 9; Length: 16; Average CI: 0.29; Sequence scores in the subdomain: 3 4 Total 1 D28 CD28 2.0 2.4 2.2 2 SKH SKH H 3.4 3.3 3.4 3 APK CAPK 1.7 1.3 1.5 4 GFR EGFR 0.3 2.1 1.3 5 FES THIS IS 1.7 4.0 3.0 6 DGM PDGF RE 7 AF1 RAF1 2.3 2.7 2.5 8 MOS CMOS 1.0 3.6 2.4 9 EE1 WEE1 1.9 2.4 2.2 10 SVK HSVK 3.6 2.0 2.7 ****************************************************** Block 3. Size: 9; Length: 7; Average CI: 0.26; 1 CONSENSUS VRILRRL Score/pos. Ins. 1 D28 CD28 54 ISLLKEL 60 2.00 0 2 SKH SKH H 49 LRVLRRV 55 3.43 0 3 APK CAPK 52 KRILQAV 58 1.71 0 4 GFR EGFR 54 AYVMASV 60 0.29 0 5 FES THIS IS 65 AKILKQY 71 1.71 0 6 DGM PDGF RE 7 AF1 RAF1 48 VAVLRKT 54 2.29 0 8 MOS CMOS 50 LNVA RL 55 1.00 0 9 EE1 WEE1 52 VSIQRAL 58 1.86 k 10 SVK HSVK 43 ARLLRRL 49 3.57 0 Block 4. Size: 9; Length: 9; Average CI: 0.30; 1 CONSENSUS RHPNIVRLM Score/pos. Ins. 1 D28 CD28 61 KDDNIVRLY 69 2.44 9 2 SKH SKH H 56 RHANIIQLV 64 3.33 8 3 APK CAPK 59 NFPFLVKLE 67 1.33 8 4 GFR EGFR 61 DNPHVCRLL 69 2.11 7 5 FES THIS IS 72 SHPNIVRLI 80 4.00 8 6 DGM PDGF RE 7 AF1 RAF1 55 RHVNILLFM 63 2.67 7 8 MOS CMOS 56 RHDNIVRVV 64 3.56 25 9 EE1 WEE1 60 GHDHIVELM 68 2.44 8 10 SVK HSVK 50 DHPAILPLL 58 2.00 18 Block 5. Size: 8; Length: 10; Average CI: 0.28; 1 CONSENSUS LYMVMELCEG Score/pos. Ins. 1 D28 CD28 79 LYLVFEFLD 87 1.80 0 2 SKH SKH H 73 VYMVMELATG 82 3.50 0 3 APK CAPK 76 LYMVMEYVPG 85 3.20 0 4 GFR EGFR 77 VQLITQLMPF 86 0.50 0 5 FES THIS IS 89 IYIVMELVQG 98 3.40 0 6 DGM PDGF RE 7 AF1 RAF1 71 LAIVTQWCEG 80 2.50 0 8 MOS CMOS 90 LHQVIYGAAG 99 1.00 3 9 EE1 WEE1 77 LYMQVELCEN 86 3.60 0 10 SVK HSVK Block 6. Size: 10; Length: 8; Average CI: 0.24; 1 CONSENSUS GDLFD-YL-R Score/pos. Ins. 1 D28 CD28 88 LDLKR YM E 95 1.12 18 2 SKH SKH H 83 GELFD RI I 90 2.62 15 3 APK CAPK 86 GEMFS HL R 93 3.38 15 4 GFR EGFR 87 GCLLD YV R 94 3.12 16 5 FES THIS IS 99 GDFLT FL R 106 2.88 16 6 DGM PDGF RE 57 GDLVD YL H 64 3.88 114 7 AF1 RAF1 81 SSLYK HL H 88 1.50 16 8 MOS CMOS 103 GDAGEpHC R 111 1.50 15 9 EE1 WEE1 87 GSLDR FL E 94 1.50 18 10 SVK HSVK 77 DLYT YLsR 84 2.50 15 ****************************************************** Blocks 7-8 are part of a Subdomain. Block 7. Length: 30; Average CI: 0.38; Block 8. Length: 10; Average CI: 0.57; Total: Size: 10; Length: 40; Average CI: 0.43; Sequence scores in the subdomain: 7 8 Total 1 D28 CD28 3.2 4.6 3.5 2 SKH SKH H 2.9 2.8 2.9 3 APK CAPK 2.9 3.0 3.0 4 GFR EGFR 2.7 3.8 3.0 5 FES THIS IS 2.3 3.7 2.7 6 DGM PDGF RE 3.3 4.0 3.5 7 AF1 RAF1 3.6 3.9 3.7 8 MOS CMOS 2.9 2.6 2.8 9 EE1 WEE1 2.7 3.6 2.9 10 SVK HSVK 2.9 2.7 2.9 ****************************************************** Block 7. Size: 10; Length: 30; Average CI: 0.38; 1 11 21 CONSENSUS QVANGMDYLH SKNIIHRDLK PENILINEPG Score/pos. Ins. 1 D28 CD28 114 QLCKGIAYCH SHRILHRDLK PQNLLINKDG 143 3.17 n 2 SKH SKH H 106 MVLDGVRYLH ALGITHRDLK PENLLYYHPG 135 2.90 4 3 APK CAPK 109 QIVLTFEYLH SLDLIYRDLK PENLLIDQQG 138 2.93 y 4 GFR EGFR 111 QIAKGMNYLE DRRLVHRDLA ARNVLVKTPQ 140 2.67 h 5 FES THIS IS 123 DAAAGMEYLE SKCCIHRDLA ARNCLVTEKN 152 2.33 v 6 DGM PDGF RE 179 QVANGMDFLA SKNCVHRDLA ARNVLICE G 207 3.30 kl 7 AF1 RAF1 105 QTAQGMDYLH AKNIIHRDMK SNNIFLHE G 133 3.57 lt 8 MOS CMOS 127 DVVNGLLFLH SQSIVHLDLK PANILISEQD 156 2.93 v 9 EE1 WEE1 113 EVALGLQFIH HKNYVHLDLK PANVMITFEG 142 2.70 t 10 SVK HSVK 100 QLLSAVDYIH RQGIIHRDIK TENIFINTPE 129 2.93 d Block 8. Size: 10; Length: 10; Average CI: 0.57; 1 CONSENSUS IKIGDFGLAR Score/pos. Ins. 1 D28 CD28 145 LKLGDFGLAR 154 4.60 16 2 SKH SKH H 140 IIITDFGLAS 149 2.80 17 3 APK CAPK 140 IQVTDFGFAK 149 3.00 13 4 GFR EGFR 142 VKITDFGLAK 151 3.80 18 5 FES THIS IS 154 LKISDFGMSR 163 3.70 18 6 DGM PDGF RE 210 VKICDFGLAR 219 4.00 18 7 AF1 RAF1 136 VKIGDFGLA 144 3.90 19 8 MOS CMOS 158 CKISDFGCSE 167 2.60 19 9 EE1 WEE1 144 LKIGDFGMA 152 3.60 15 10 SVK HSVK 131 ICLGDFGAAC 140 2.70 18 Block 9. Size: 10; Length: 6; Average CI: 0.51; 1 CONSENSUS EW-MAPE Score/pos. Ins. 1 D28 CD28 171 WyRAPE 176 2.50 7 2 SKH SKH H 167 EY IAPE 172 3.83 6 3 APK CAPK 163 EY LAPE 168 4.00 6 4 GFR EGFR 170 KW MALE 175 3.67 6 5 FES THIS IS 182 KW TAPE 187 4.17 6 6 DGM PDGF RE 238 KW MAPE 243 5.17 6 7 AF1 RAF1 164 LW MAPE 169 4.50 9 8 MOS CMOS 187 TH RAPE 192 1.83 6 9 EE1 WEE1 168 EY IAPE 173 3.83 6 10 SVK HSVK 159 DT NAPE 164 2.17 6 Block 10. Size: 10; Length: 17; Average CI: 0.40; 1 11 CONSENSUS YTTQADVWSF GIVIWEM Score/pos. Ins. 1 D28 CD28 184 YSTGVDTWSI GCIFAEM 200 2.65 cn 2 SKH SKH H 179 YTNSVDMWAL GVIAYIL 195 2.41 3 3 APK CAPK 175 YNKAVDWWAL GVLIYEM 191 2.59 0 4 GFR EGFR 182 YTHQSDVWSY GVTVWEL 198 4.18 4 5 FES THIS IS 194 YSSESDVWSF GILLWET 210 4.00 4 6 DGM PDGF RE 250 YTTLSDVWSF GILLWEI 266 4.35 4 7 AF1 RAF1 179 FSFQSDVYSY GIVLYEL 195 3.06 3 8 MOS CMOS 199 VTPKADIYSF AITLWQM 215 3.06 3 9 EE1 WEE1 180 YDKPADIFSL GITVFEA 196 2.29 0 10 SVK HSVK 171 YTTTVDIWSA GLVIFET 187 3.35 8 Block 11. Size: 10; Length: 4; Average CI: 0.22; 1 CONSENSUS SAPY Score/pos. Ins. 1 D28 CD28 203 RKPI 206 0.75 8 2 SKH SKH H 199 TMPF 202 2.25 6 3 APK CAPK 192 AAGY 195 2.25 9 4 GFR EGFR 203 SKPY 206 3.75 6 5 FES THIS IS 215 ASPY 218 3.50 6 6 DGM PDGF RE 271 GTPY 274 3.00 7 7 AF1 RAF1 199 ELPY 202 2.75 3 8 MOS CMOS 219 QAPY 222 4.00 6 9 EE1 WEE1 197 AANI 200 0.50 6 10 SVK HSVK 196 SAPR 199 3.00 18 Block 12. Size: 10; Length: 3; Average CI: 0.22; 1 CONSENSUS QIY Score/pos. Ins. 1 D28 CD28 215 QIF 217 4.00 50 2 SKH SKH H 209 RLY 211 3.00 15 3 APK CAPK 205 QIY 207 5.00 48 4 GFR EGFR 213 EIS 215 1.33 11 5 FES THIS IS 225 QTR 227 0.67 11 6 DGM PDGF RE 282 QFY 284 3.67 11 7 AF1 RAF1 206 QII 208 2.67 9 8 MOS CMOS 229 ILY 231 1.67 8 9 EE1 WEE1 207 QSW 209 1.67 11 10 SVK HSVK 218 QVH 220 3.33 7 Block 13. Size: 8; Length: 3; Average CI: 0.37; 1 CONSENSUS PEL Score/pos. Ins. 1 D28 CD28 2 SKH SKH H 227 PSV 229 2.33 23 3 APK CAPK 4 GFR EGFR 227 PPI 229 2.33 24 5 FES THIS IS 239 PEL 241 5.00 27 6 DGM PDGF RE 296 PAH 298 0.67 30 7 AF1 RAF1 218 PDL 220 4.00 36 8 MOS CMOS 240 PSL 242 3.33 31 9 EE1 WEE1 221 PRL 223 3.33 27 10 SVK HSVK 228 PES 230 3.00 23 Block 14. Size: 9; Length: 3; Average CI: 0.24; 1 CONSENSUS ERL Score/pos. Ins. 1 D28 CD28 268 DKL 270 2.67 23 2 SKH SKH H 253 QAL 255 1.67 8 3 APK CAPK 4 GFR EGFR 254 ELI 256 1.67 8 5 FES THIS IS 269 QEL 271 2.00 0 6 DGM PDGF RE 329 ERL 331 4.67 9 7 AF1 RAF1 257 ELL 259 2.33 qh 8 MOS CMOS 274 ARL 276 2.67 10 9 EE1 WEE1 251 DRV 253 2.67 27 10 SVK HSVK 254 TRY 256 1.00 7 *********************************************** SIMILARITY MATRICES WITHIN BLOCKS OF ALIGNED SEQUENCES. Similarities are evaluated normalizing the sum of the scores of the aligned positions within the block by the minimum self-score of block sequences. Block 1. Size: 9; Length: 12; Average CI: 0.50; Similarities between sequences. 1 2 3 4 5 6 7 8 9 1 D28 CD28 - 37 34 61 48 48 66 59 36 2 SKH SKH H - 61 37 47 51 46 40 54 3 APK CAPK ...... - 46 42 57 47 47 49 4 GFR EGFR - 56 67 92 74 49 5 FES THIS IS ............ - 48 65 52 53 6 DGM PDGF RE - 59 64 55 7 AF1 RAF1 .................. - 70 47 8 MOS CMOS - 49 9 EE1 WEE1 ........................ - Average similarity: 53.1 Block 2. Size: 9; Length: 14; Average CI: 0.10; Similarities between sequences. 1 2 3 4 5 6 7 9 10 1 D28 CD28 - 2 6 8 6 29 0 22 0 2 SKH SKH H - 15 32 0 17 7 10 0 3 APK CAPK ...... - 13 0 10 10 13 0 4 GFR EGFR - 24 10 19 31 12 5 FES THIS IS ............ - 13 13 19 14 6 DGM PDGF RE - 17 21 5 7 AF1 RAF1 .................. - 6 10 9 EE1 WEE1 - 10 10 SVK HSVK ........................ - Average similarity: 11.8 Block 3. Size: 9; Length: 7; Average CI: 0.26; Similarities between sequences. 1 2 3 4 5 7 8 9 10 1 D28 CD28 - 30 10 0 37 37 26 40 40 2 SKH SKH H - 45 16 32 48 48 20 65 3 APK CAPK ...... - 19 26 13 0 17 35 4 GFR EGFR - 16 10 0 7 13 5 FES THIS IS ............ - 23 4 7 45 7 AF1 RAF1 - 11 30 32 8 MOS CMOS .................. - 26 30 9 EE1 WEE1 - 23 10 SVK HSVK ........................ - Average similarity: 24.5 Block 4. Size: 9; Length: 9; Average CI: 0.30; Similarities between sequences. 1 2 3 4 5 7 8 9 10 1 D28 CD28 - 37 7 22 45 13 58 33 2 2 SKH SKH H - 7 16 60 53 67 42 28 3 APK CAPK ...... - 13 27 0 4 13 16 4 GFR EGFR - 48 0 18 29 42 5 FES THIS IS ............ - 32 64 45 45 7 AF1 RAF1 - 40 22 18 8 MOS CMOS .................. - 49 16 9 EE1 WEE1 - 29 10 SVK HSVK ........................ - Average similarity: 29.4 Block 5. Size: 8; Length: 10; Average CI: 0.28; Similarities between sequences. 1 2 3 4 5 7 8 9 1 D28 CD28 - 36 53 18 44 22 0 33 2 SKH SKH H - 63 17 70 17 25 40 3 APK CAPK ...... - 19 66 27 22 31 4 GFR EGFR - 19 17 0 0 5 FES THIS IS ............ - 30 11 38 7 AF1 RAF1 - 8 27 8 MOS CMOS .................. - 0 9 EE1 WEE1 - Average similarity: 26.9 Block 6. Size: 10; Length: 8; Average CI: 0.24; Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 0 0 0 10 25 15 0 35 38 2 SKH SKH H - 45 25 8 35 13 5 3 13 3 APK CAPK ...... - 29 40 36 39 36 18 48 4 GFR EGFR - 36 49 10 14 18 28 5 FES THIS IS ............ - 45 2 19 28 55 6 DGM PDGF RE - 37 26 30 45 7 AF1 RAF1 .................. - 7 25 38 8 MOS CMOS - 5 18 9 EE1 WEE1 ........................ - 18 10 SVK HSVK - Average similarity: 23.8 Block 7. Size: 10; Length: 30; Average CI: 0.38; Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 43 48 41 28 36 46 45 38 42 2 SKH SKH H - 45 30 19 31 43 39 31 42 3 APK CAPK ...... - 28 28 34 41 42 39 38 4 GFR EGFR - 50 55 42 32 30 42 5 FES THIS IS ............ - 60 40 37 31 23 6 DGM PDGF RE - 56 43 42 30 7 AF1 RAF1 .................. - 40 41 49 8 MOS CMOS - 55 37 9 EE1 WEE1 ........................ - 28 10 SVK HSVK - Average similarity: 39.1 Block 8. Size: 10; Length: 10; Average CI: 0.57; Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 51 52 69 76 72 89 48 89 54 2 SKH SKH H - 66 74 51 60 62 40 54 49 3 APK CAPK ...... - 80 57 59 60 43 57 33 4 GFR EGFR - 69 82 82 57 70 37 5 FES THIS IS ............ - 71 69 67 80 33 6 DGM PDGF RE - 80 48 67 33 7 AF1 RAF1 .................. - 58 89 64 8 MOS CMOS - 57 23 9 EE1 WEE1 ........................ - 61 10 SVK HSVK - Average similarity: 60.9 Block 9. Size: 10; Length: 6; Average CI: 0.51; Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 47 50 45 69 69 71 56 47 41 2 SKH SKH H - 93 33 57 63 50 43100 40 3 APK CAPK ...... - 37 57 67 53 47 93 40 4 GFR EGFR - 61 79 58 6 33 3 5 FES THIS IS ............ - 83 65 34 57 38 6 DGM PDGF RE - 82 34 63 31 7 AF1 RAF1 .................. - 34 50 22 8 MOS CMOS - 43 38 9 EE1 WEE1 ........................ - 40 10 SVK HSVK - Average similarity: 51.6 Block 10. Size: 10; Length: 17; Average CI: 0.40; Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 38 46 29 38 40 30 11 24 48 2 SKH SKH H - 58 45 37 42 31 16 28 48 3 APK CAPK ...... - 39 43 43 33 18 45 47 4 GFR EGFR - 68 70 65 52 47 48 5 FES THIS IS ............ - 78 61 46 42 55 6 DGM PDGF RE - 56 49 36 57 7 AF1 RAF1 .................. - 40 39 39 8 MOS CMOS - 34 25 9 EE1 WEE1 ........................ - 46 10 SVK HSVK - Average similarity: 42.9 Block 11. Size: 10; Length: 4; Average CI: 0.22; Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 20 0 45 20 10 15 25 0 5 2 SKH SKH H - 0 43 40 27 48 33 0 16 3 APK CAPK ...... - 20 45 20 10 35 39 5 4 GFR EGFR - 65 52 48 52 0 37 5 FES THIS IS ............ - 65 45 60 11 32 6 DGM PDGF RE - 43 48 0 21 7 AF1 RAF1 .................. - 62 0 16 8 MOS CMOS - 0 42 9 EE1 WEE1 ........................ - 0 10 SVK HSVK - Average similarity: 27.1 Block 12. Size: 10; Length: 3; Average CI: 0.22; Similarities between sequences. 1 2 3 4 5 6 7 8 9 10 1 D28 CD28 - 40 80 31 7 53 69 14 27 47 2 SKH SKH H - 60 0 0 47 15 50 7 27 3 APK CAPK ...... - 31 13 73 62 36 33 67 4 GFR EGFR - 0 0 31 0 0 31 5 FES THIS IS ............ - 7 8 0 20 33 6 DGM PDGF RE - 31 21 29 38 7 AF1 RAF1 .................. - 0 0 38 8 MOS CMOS - 0 0 9 EE1 WEE1 ........................ - 6 10 SVK HSVK - Average similarity: 26.3 Block 13. Size: 8; Length: 3; Average CI: 0.37; Similarities between sequences. 2 4 5 6 7 8 9 10 2 SKH SKH H - 57 50 29 50 79 43 29 4 GFR EGFR - 47 13 44 50 40 20 5 FES THIS IS ...... - 13 80 71 67 60 6 DGM PDGF RE - 6 29 13 27 7 AF1 RAF1 ............ - 71 53 40 8 MOS CMOS - 64 29 9 EE1 WEE1 .................. - 27 10 SVK HSVK - Average similarity: 42.9 Block 14. Size: 9; Length: 3; Average CI: 0.24; Similarities between sequences. 1 2 4 5 6 7 8 9 10 1 D28 CD28 - 23 15 36 57 31 31 60 0 2 SKH SKH H - 23 62 38 38 15 0 0 4 GFR EGFR ...... - 8 38 85 0 23 0 5 FES THIS IS - 43 23 23 7 0 6 DGM PDGF RE ............ - 54 62 57 21 7 AF1 RAF1 - 8 8 0 8 MOS CMOS .................. - 31 31 9 EE1 WEE1 - 20 10 SVK HSVK ........................ - Average similarity: 27.0 *********************************************** BLOCKS OF ALIGNED SEQUENCES: Consensus and Conservation Index '>' indicates an insertion length of at least 10 residues. The length of shorter insertions is explicitly indicated column by column (each colum is a sequence). 8888>8888 1 0.67 L |VILLILILL | Block 1 2 1.00 G |GGGGGGGGG | 3 0.16 S |ERTSRSSAS | 4 1.00 G |GGGGGGGGG | 5 0.13 S |TSSANASGE | 6 0.89 F |YFFFFFFFF | 7 0.61 G |GSGGGGGGS | 8 -0.03 x |VRRTEQTSE | 9 1.00 V |VVVVVVVVV | 10 0.26 Y |YVMYFVYYF | 11 0.16 K |KRLKSEKKQ | 12 0.10 A |AVVGGAGAV | >>>>>>>>> 1 0.27 E |-EKEESD KD| Block 2 2 -0.09 x |V-VATTP FS| 3 0.12 T |ATVTLAT SS| 4 -0.21 x |LKKSPR- GH| 5 0.08 P |-YLPPSP PP| 6 0.21 K |KRKKDSE KD| 7 -0.01 x |-EQAIEQ EY| 8 -0.21 x |KGINKKF RP| 9 -0.02 x |IREKAQQ NQ| 10 0.08 R |REHEKAA RR| 11 0.20 L |LVTIFLF LV| 12 0.04 L |ECLLLMR LI| 13 0.06 N |SSNDQSN QV| 14 0.82 E |EEEEEEE EK| >000020 0> 1 0.09 V |ILKAA VLVA| Block 3 2 0.01 R |SRRYK ANSR| 3 0.67 I |LVIVI VVIL| 4 0.36 L |LLLML LAQL| 5 0.35 R |KRQAK R-RR| 6 0.10 R |ERASQ KRAR| 7 0.26 L |LVVVY TLLL| 00000 0010 1 0.02 R |KRNDS RRGD| Block 4 2 0.37 H |DHFNH HHHH| 3 0.04 P |DAPPP VDDP| 4 0.18 N |NNFHN NNHA| 5 0.83 I |IILVI IIII| 6 0.47 V |VIVCV LVVL| 7 0.09 R |RQKRR LREP| 8 0.63 L |LLLLL FVLL| 9 0.12 M |YVELI MVML| 98878 7>8> 1 0.62 L |LVLVI LLL | Block 5 2 0.29 Y |YYYQY AHY | 3 0.31 M |LMMLI IQM | 4 0.58 V |VVVIV VVQ | 5 0.13 M |FMMTM TIV | 6 0.46 E |EEEQE QYE | 7 0.01 L |FLYLL WGL | 8 0.07 C |LAVMV CAC | 9 -0.02 x |DTPPQ EAE | 10 0.33 G |-GGFG GGN | 00000 030 1 0.41 G |LGGGGGSGG-| Block 6 2 0.26 D |DEECDDSDSD| 3 0.48 L |LLMLFLLALL| 4 -0.19 x |KFFLLVYGDY| 5 0.04 D |RDSDTDKERT| 6 0.32 Y |YRHYFYHHFY| 7 0.48 L |MILVLLLCLL| 8 0.14 R |EIRRRHHRER| >>>>>>>>>> 1 0.39 Q |QMQQDQQDEQ| Block 7 2 0.36 V |LVIIAVTVVL| 3 0.22 A |CLVAAAAVAL| 4 -0.14 x |KDLKANQNLS| 5 0.56 G |GGTGGGGGGA| 6 0.39 M |IVFMMMMLLV| 7 -0.03 x |ARENEDDLQD| 8 0.77 Y |YYYYYFYFFY| 9 0.61 L |CLLLLLLLII| 10 0.42 H |HHHEEAHHHH| 11 0.20 S |SASDSSASHR| 12 0.08 K |HLLRKKKQKQ| 13 -0.01 x |RGDRCNNSNG| 14 0.28 I |IILLCCIIYI| 15 0.58 I |LTIVIVIVVI| 16 0.86 H |HHYHHHHHHH| 17 0.49 R |RRRRRRRLLR| 18 1.00 D |DDDDDDDDDD| 19 0.80 L |LLLLLLMLLI| 20 0.43 K |KKKAAAKKKK| 21 0.20 P |PPPAAASPPT| 22 0.11 E |QEERRRNAAE| 23 1.00 N |NNNNNNNNNN| 24 0.46 I |LLLVCVIIVI| 25 0.56 L |LLLLLLFLMF| 26 0.59 I |IYIVVILIII| 27 -0.09 x |NYDKTCHSTN| 28 0.05 E |KHQTEEEEFT| 29 0.10 P |DPQPK--QEP| 30 0.23 G |GGGQNGGDGE| 1411122111 1 0.46 I |LIIVLVVCLI| Block 8 2 0.29 K |KIQKKKKKKC| 3 0.77 I |LIVIIIIIIL| 4 0.10 G |GTTTSCGSGG| 5 1.00 D |DDDDDDDDDD| 6 1.00 F |FFFFFFFFFF| 7 1.00 G |GGGGGGGGGG| 8 0.26 L |LLFLMLLCMA| 9 0.73 A |AAAASAASAA| 10 0.11 R |RSKKRR-E-C| >>>>>>>>>> 1 0.07 E |-EEKKKLTED| Block 9 2 0.31 W |WYYWWWWHYT| 3 -0.02 x |RILMTMMRIN| 4 1.00 A |AAAAAAAAAA| 5 0.68 P |PPPLPPPPPP| 6 1.00 E |EEEEEEEEEE| 7666669666 1 0.67 Y |YYYYYYFVYY| Block 10 2 0.36 T |STNTSTSTDT| 3 -0.08 x |TNKHSTFPKT| 4 -0.15 x |GSAQELQKPT| 5 0.16 A |VVVSSSSAAV| 6 1.00 D |DDDDDDDDDD| 7 0.33 V |TMWVVVVIII| 8 0.61 W |WWWWWWYYFW| 9 0.73 S |SAASSSSSSS| 10 0.15 F |ILLYFFYFLA| 11 0.80 G |GGGGGGGAGG| 12 0.58 I |CVVVIIIIIL| 13 0.27 V |IILTLLVTTV| 14 0.33 I |FAIVLLLLVI| 15 0.29 W |AYYWWWYWFF| 16 0.56 E |EIEEEEEQEE| 17 0.19 M |MLMLTILMAT| 2304443308 1 0.05 S |RTASAGEQAS| Block 11 2 0.05 A |KMAKSTLAAA| 3 0.50 P |PPGPPPPPNP| 4 0.28 Y |IFYYYYYYIR| 869667366> 1 0.43 Q |QRQEQQQIQQ| Block 12 2 0.20 I |ILIITFILSV| 3 0.04 Y |FYYSRYIYWH| >>>>>>98>7 1 1.00 P | P PPPPPPP| Block 13 2 -0.01 x | S PEADSRE| 3 0.11 L | V ILHLLLS| > >>>>>>> 1 0.17 E |DQ EQEEADT| Block 14 2 0.03 R |KA LERLRRR| 3 0.52 L |LL ILLLLVY| >8 8092>>7 Total time after optimizations = 3' 11"